Determination of the Significance of the Most Effective Nutrients on Lipase Production from Cryptococcus albidus D24

In this study, the optimization of medium components used for production lipase enzyme from Cryptococcus albidus D24 was performed using Plackett-Burman statistical design method (PBD), and most important nutrients affecting strain were determined as first step. According to PBD, highest activity (19.34 U/ml/min) obtained with including Tween 80 (X2) 2.5% (v/v), (g/L) Peptone (X4) 8.0, Yeast Extract (X6) 7.5, Beef (X7) Malt (X8) NH4Cl (X9) 6.0, NaNO3 (X10) 1.5, (NH4)NO3 (X12) (NH4)HCO3(X13) MgSO4.7H2O (X15) 1.0, KH2PO4 (X16) 2.0 at end 144 h cultivation. Regarding concentration effect (CE) values (CE=7.1587), olive oil (CE=3.5544), (NH4)HCO3 (CE=3.0747), tryptone (CE=2.1427) evaluated more effective among sixteen compounds studied. After that, optimum concentrations these experimented Response Surface Methodology (RSM). Experimental results showed that containing (X3) 1.5% (X5) 3.0, (X13) yielded maximum (12.03 compared other studied compounds. Although RSM methodology lower than by cost produce one-unit is 0.104 Euro in while only 0.0277 spent RSM. words, coded X3, X5, X9, X13 provides a cost-effective process.